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Figure 2.8 Solid Phase RNA Synthesis. 5 -dimethoxytrityl (DMT)-deprotection of resin bound 3 terminal nucleoside residue is effected with trichloroacetic acid (TCA) (see Fig. 2.5). Thereafter the rst coupling reaction is enabled by phosphoamidite activation with tetrazole (see Fig. 2.5) followed by oxidation of the newly formed diester linkage to a phosphodiester link. The process of 5 -DMT deprotection, phosphoramidite coupling and then diester oxidation, continues for as many times as required (n-times), prior to global deprotection and resin removal under basic conditions. RNA synthesis requires that 2 -hydroxyl groups are protected during the synthesis by tert-butyl dimethyl silyl (TBDMS) protecting groups labile only to uoride treatment from tetra butyl ammonium uoride (TBAF) (mechanism shown).

By comparison, the standard SSI translator s getTranslatorInfo() function has 10 array elements, and Webmonkey s XSSI has 17.

The principle of relativity requires that under a Lorentz transformation t o a primed coordinate system the Dirac equation retain the same form:

See Photoshop CS5 Bible, by Lisa DaNae Dayley and Brad Dayley, for a more detailed description of layer blend modes in Photoshop.

Changing menus can be a bad idea. You might forget about a feature that you later want to use, for example. People sharing a computer may get confused when the interface looks different than they are used to and they can t find the commands they re used to. n

The rst three lines are now:

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To protect auto-entered values, you might want to select the Prohibit modification of value during data entry check box near the bottom of the Options dialog. Setting this option prevents you and other users of the database from inadvertently altering the contents of an auto-entry field. You need to set this option for each field you want to protect (a serial number field, for instance). If you later discover that you need to edit one or more of the auto-entered values for a particular field, return to this screen, remove the check mark from the Prohibit modification of value during data entry check box, edit the field contents as necessary, and then restore the check mark.

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Moving from page to page in a long document and scrolling around a large or magnified page are among the most common tasks you ll perform in InDesign. The more time you spend displaying the page or page area you want to work on, the less time you have to do the work you need to do. As with most trips, the less time you spend between destinations, the better. You can always just go to the page you want, but for navigating through the pages of a document when you don t know which page has the content you are seeking, the Pages panel (choose Window Pages or press +F12 or Ctrl+F12) offers the fastest ride. For navigating around in a page, you may want to switch to the navigator function, described in 3.

After the characters are designed and the voice talent has been hired, the dialog can be recorded.The sooner this is done, the better, because the animators can't move very far along without it. Some studios start out with a scratch track (placeholder soundtrack) of voices recorded in-house to use as a placeholder until the final recording is available.

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